Histone 3 抗体 (H3K27ac)
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- 抗原 See all Histone 3 (H3) 抗体
- Histone 3 (H3)
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抗原表位
- H3K27ac
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Histone 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Peptide Array (PeA)
- 特异性
- Polyclonal antibody raised in rabbit against the region of histone H3 containing the acetylated lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.
- 交叉反应
- Arabidopsis, 小鼠, 大鼠
- 交叉反应 (详细)
- wide range
- 纯化方法
- Peptide affinity purified
- 质量等级
- ChIP-seq Grade
- 免疫原
- synthetic peptide
- Top Product
- Discover our top product H3 Primary Antibody
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- 应用备注
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ChIP/ChIP-seq 1 μg:ChIP
ELISA 1:500
Dot Blot 1:20,000
WB 1:1,000
IF 1:500 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.8 μg/μL
- 缓冲液
- PBS, 0.05 % azide, 0.05 % ProClin300
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
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- 抗原
- Histone 3 (H3)
- 别名
- Histone 3 (H3 产品)
- 别名
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
- 背景
- Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Acetylation of histone H3K27 is associated with active promoters and enhancers.
- UniProt
- P68431
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