CHAF1B 抗体
-
- 抗原 See all CHAF1B 抗体
- CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 单克隆
-
标记
- This CHAF1B antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))
- 交叉反应
- 人
- 纯化方法
- Purified by Protein A.
- 免疫原
- Recombinant protein corresponding to the C-terminus of Human p60 CAF1.
- 克隆位点
- 9A7
- 亚型
- IgG
- Top Product
- Discover our top product CHAF1B Primary Antibody
-
-
- 应用备注
-
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg) - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C for 12 months.
- 有效期
- 12 months
-
- 抗原
- CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))
- 别名
- CHAF1B (CHAF1B 产品)
- 别名
- CHAF1B antibody, wu:fd07c09 antibody, wu:fe36g06 antibody, wu:fv38g09 antibody, zgc:56096 antibody, CAF-1 antibody, CAF-IP60 antibody, CAF1 antibody, CAF1A antibody, CAF1P60 antibody, MPHOSPH7 antibody, MPP7 antibody, 2600017H24Rik antibody, C76145 antibody, CAF-I p60 antibody, CAF-Ip60 antibody, caf-1 antibody, CAF-1P60 antibody, chromatin assembly factor 1 subunit B antibody, chromatin assembly factor 1, subunit B antibody, chromatin assembly factor 1, subunit B (p60) antibody, chromatin assembly factor 1 subunit B L homeolog antibody, CHAF1B antibody, chaf1b antibody, Chaf1b antibody, chaf1b.L antibody
- 背景
-
Synonyms: Chromatin assembly factor 1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7, p60 CAF1
Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin-associated proteins 1 (HP1_, HP1_ and HP1_). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
- 基因ID
- 8208
- UniProt
- Q13112
-