CDT1 抗体
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- 抗原 See all CDT1 抗体
- CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This CDT1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人
- 交叉反应 （详细）
- WB(1:500-1000), IHC-P(1:25-50)
- 纯化方法
- Purified by Protein A.
- 免疫原
- Human CDT1 aa 100 to the C-terminus
- 克隆位点
- 2H5
- 亚型
- IgG
- Top Product
- Discover our top product CDT1 Primary Antibody
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anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) antibody
CDT1 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (C-Term) antibodyVerified CDT1 适用: 人 ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (C-Term) antibodyCDT1 适用: 人, 小鼠, 大鼠, Pig, 兔, Saccharomyces cerevisiae, Cow, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (AA 12-170) antibodyCDT1 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (C-Term) antibodyCDT1 适用: 人, 小鼠, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (AA 400-449) antibodyCDT1 适用: 人, 小鼠, 大鼠, Pig, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Chromatin Licensing and DNA Replication Factor 1 (CDT1) (C-Term) antibodyCDT1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB 1:300-5000
IHC-P 1:200-400 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C for 12 months.
- 有效期
- 12 months
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- 抗原
- CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))
- 别名
- CDT1 (CDT1 产品)
- 背景
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Synonyms: DNA replication factor Cdt1, Double parked homolog, DUP, CDT1, chromatin licensing and DNA replication factor 1.
Background: Human Cdt1 is a nuclear localizing replication initiation factor that is expressed only during the G1 and S phases of the cell cycle. In conjunction with Cdc18, Cdt1 is required to load the MCM protein Cdc21 onto chromatin at the end of mitosis which is necessary to initiate DNA replication. After S-phase onset, Cdt1 protein levels decrease and are barely detectable in cells in early S-phase or G2. However, Cdt1 mRNA is expressed in S-phase-arrested cells, and its levels do not change dramatically during the cell cycle, suggesting that proteolytic degradation rather than transcriptional controls ensure proper accumulation of Cdt1. Cdt1 can associate with the DNA replication inhibitor geminin, which is present in the S and G2 phases of the cell cycle. Inhibition of DNA replication by geminin in cell-free DNA replication extracts can be reversed by the addition of excess Cdt1. Geminin may be responsible for preventing inappropriate origin firing by targeting Cdt1.
- 基因ID
- 81620
- UniProt
- Q9H211
- 途径
- MAPK Pathway, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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