Keratin 77 抗体
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- 抗原 See all Keratin 77 (KRT77) 抗体
- Keratin 77 (KRT77)
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适用
- 人, 大鼠, Cow, 犬, 小鼠, 兔, 小鸡, 猴, Turtle
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 77 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Human epidermal keratin
- 克隆位点
- AE-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KRT77 Primary Antibody
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anti-Keratin 77 (KRT77) antibody
KRT77 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTL-1077 unconjugated
anti-Keratin 77 (KRT77) (Internal Region) antibodyKRT77 适用: 人, 大鼠 IHC, ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Keratin 77 (KRT77) antibodyKRT77 适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTL-1577R unconjugated Recombinant Antibody
anti-Keratin 77 (KRT77) (AA 1-578) antibodyKRT77 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: Skin or squamous cell carcinoma (IHC).
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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