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Keratin 77 抗体

KRT77 适用: 人, 大鼠, Cow, 犬, 小鼠, 兔, 小鸡, 猴, Turtle IHC, WB, IF, FACS, StM 宿主: 小鼠 Monoclonal AE-1 unconjugated
产品编号 ABIN6941429
发货至: 中国
  • 抗原 See all Keratin 77 (KRT77) 抗体
    Keratin 77 (KRT77)
    适用
    • 25
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 大鼠, Cow, 犬, 小鼠, 兔, 小鸡, 猴, Turtle
    宿主
    • 18
    • 7
    小鼠
    克隆类型
    • 16
    • 9
    单克隆
    标记
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 77 antibody is un-conjugated
    应用范围
    • 10
    • 9
    • 6
    • 5
    • 4
    • 3
    • 2
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
    特异性
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.
    纯化方法
    Purified by Protein A/G
    免疫原
    Human epidermal keratin
    克隆位点
    AE-1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product KRT77 Primary Antibody
  • 应用备注

    Positive Control: Skin or squamous cell carcinoma (IHC).

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    Keratin 77 (KRT77)
    别名
    KRT77 (KRT77 产品)
    别名
    K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody
    分子量
    40-56.5kDa
    基因ID
    374454
    UniProt
    Q7Z794
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