Cytokeratin 2 抗体
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- 抗原 See all Cytokeratin 2 (KRT76) 抗体
- Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cytokeratin 2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Solubilized keratin extract from human stratum corneum
- 克隆位点
- 34BE12
- 亚型
- IgG1 kappa
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anti-Cytokeratin-2P (KRT76) antibody
KRT76 适用: 人, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Cytokeratin-2P (KRT76) (AA 173-283) antibodyKRT76 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, StM 宿主: 兔 Monoclonal KRTH-2147R unconjugated Recombinant Antibody
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人 IHC, StM 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cytokeratin-2P (KRT76) antibodyKRT76 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal rKRTH-2148 unconjugated Recombinant Antibody
anti-Cytokeratin-2P (KRT76) (AA 1-638) antibodyKRT76 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Positive Control: HeLa, MCF-7, PC12 cells, Skin, Prostate carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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