Recombinant MHC Class II HLA-DP/DQ/DR 抗体
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- 抗原 See all MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR) products
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MHC Class II HLA-DP/DQ/DR antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Coating (Coat), ELISA, Staining Methods (StM)
- 特异性
- Reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36 kDa) and a beta chain (27 kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Non-T, non-B human acute lymphoblastic leukemia REH6 cells
- 克隆位点
- RHLA-Pan-3475
- 亚型
- IgG1 kappa
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- 应用备注
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Positive Control: Raji cells. Tonsil or lymph node.
Known Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL). ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- MHC Class II HLA-DP/DQ/DR (HLA-DP/DQ/DR)
- 别名
- HLA-DP, HLA-DQ, HLA-DR (HLA-DP/DQ/DR 产品)
- 分子量
- 33-35kDa
- 基因ID
- 3115, 3117, 3122
- UniProt
- P04440, P01908, P01909, P01920, P01903
- 途径
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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