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BrdU 抗体

BrdU IHC, FACS, IF, Neut, StM 宿主: 小鼠 Monoclonal SPM166 unconjugated
产品编号 ABIN6941366
发货至: 中国
  • 抗原 See all BrdU 抗体
    BrdU (Bromodeoxyuridine (BrdU))
    适用
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    宿主
    • 42
    • 23
    • 9
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    小鼠
    克隆类型
    • 50
    • 26
    单克隆
    标记
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    This BrdU antibody is un-conjugated
    应用范围
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    Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Neutralization (Neut), Staining Methods (StM)
    特异性
    It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
    纯化方法
    Purified by Protein A/G
    免疫原
    Bromodeoxyuridine (BrdU) conjugated to KLH
    克隆位点
    SPM166
    亚型
    IgG1 kappa
    Top Product
    Discover our top product BrdU Primary Antibody
  • 应用备注

    Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Use Ab at 1 μg/mL for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    BrdU (Bromodeoxyuridine (BrdU))
    Abstract
    BrdU 产品
    物质类
    Chemical
    分子量
    307Da
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