BrdU 抗体
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- 抗原 See all BrdU 抗体
- BrdU (Bromodeoxyuridine (BrdU))
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This BrdU antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Neutralization (Neut), Staining Methods (StM)
- 特异性
- It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Bromodeoxyuridine (BrdU) conjugated to KLH
- 克隆位点
- SPM166
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product BrdU Primary Antibody
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- 应用备注
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Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (Use Ab at 1 μg/mL for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- BrdU (Bromodeoxyuridine (BrdU))
- Abstract
- BrdU 产品
- 物质类
- Chemical
- 分子量
- 307Da
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