Keratin 7/17 抗体
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- 抗原 See all Keratin 7/17 (KRT7/17) 抗体
- Keratin 7/17 (KRT7/17)
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适用
- 人, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Keratin 7/17 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
- 特异性
- It recognizes intermediate filament proteins (IFP's) of 55 kDa and 46 kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation.
- 无交叉反应
- 雪貂, 小鼠, 兔, 大鼠
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)
- 克隆位点
- C-46
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product KRT7/17 Primary Antibody
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anti-Keratin 7/17 (KRT7/17) antibody
KRT7/17 适用: 人, Cow, Pig WB, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow, Pig WB, IHC, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Positive Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Keratin 7/17 (KRT7/17)
- 别名
- KRT7 & KRT17 (KRT7/17 产品)
- 别名
- KRT7 antibody, CK7 antibody, keratin 7 antibody, KRT7 antibody
- 分子量
- 55kDa (CK7), 46kDa (CK17)
- 基因ID
- 3855, 3872
- UniProt
- P08729, Q04695
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