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- 抗原 See all Biotin products
- Biotin
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Biotin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Confocal Microscopy (CM)
- 特异性
- It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Biotinylated sheep immunoglobulin
- 克隆位点
- Hyb-8
- 亚型
- IgG1 kappa
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anti-Biotin antibody
WB, ELISA, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyELISA 宿主: 山羊 Polyclonal unconjugated Fab fragment
anti-Biotin antibody (DyLight 488)WB, FM, FLISA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibodyFACS, ELISA 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyFACS, ELISA, IP, CyTOF 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
anti-Biotin antibodyIP, Con 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Positive Control: Biotinylated proteins in solution or on tissues.
Known Application: Confocal Microscopy, Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), In situ Nucleic Acid Hybridization, Western Blot, Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Biotin
- 别名
- Biotin (Vitamin B7 or Vitamin H) (Biotin 产品)
- 物质类
- Chemical
- 分子量
- 244Da
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