CDC20 抗体
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- 抗原 See all CDC20 抗体
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CDC20 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Urea-denatured His6 Cdc20 human recombinant protein
- 克隆位点
- AR12
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CDC20 Primary Antibody
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- 应用备注
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Positive Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
- 别名
- CDC20 (CDC20 产品)
- 别名
- X-FZY antibody, ba276h19.3 antibody, cdc20a antibody, fizzy1 antibody, p55cdc antibody, CDC20A antibody, bA276H19.3 antibody, p55CDC antibody, 2310042N09Rik antibody, C87100 antibody, cell division cycle 20 antibody, cell division cycle 20 L homeolog antibody, cell division cycle 20 homolog (S. cerevisiae) antibody, cdc20 antibody, CDC20 antibody, cdc20.L antibody, Cdc20 antibody
- 背景
- Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
- 分子量
- 55kDa
- 基因ID
- 991
- UniProt
- Q12834
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