CD68 抗体 (AA 150-301)
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- 抗原 See all CD68 抗体
- CD68 (CD68 Molecule (CD68))
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抗原表位
- AA 150-301
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD68 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunostaining (ISt), Staining Methods (StM), Coating (Coat)
- 特异性
- This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant fragment of humanCD68 protein (around aa 150-301) (exact sequence is proprietary)
- 克隆位点
- LAMP4-1830
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CD68 Primary Antibody
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- 应用备注
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Positive Control: U87MG, Tonsil, Lymph Node or Spleen.
Known Application: Flow Cytometry (1-2 μg/million cells),ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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