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- 抗原 See all CD53 抗体
- CD53
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD53 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)
- 特异性
- Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop V, Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Human Sezary cells
- 克隆位点
- 63-5A3
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CD53 Primary Antibody
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anti-CD53 (CD53) antibody
CD53 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7A8D3 unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1H2C7 unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 PE
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal MEM-53 unconjugated
anti-CD53 (CD53) (AA 100-180) antibodyCD53 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibodyCD53 适用: 人 FACS, WB, IF, IP, IHC 宿主: 兔 Chimeric HD77 unconjugated Recombinant Antibody
anti-CD53 (CD53) (Internal Region) antibodyCD53 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibody (FITC)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-53 FITC
anti-CD53 (CD53) (AA 65-160) antibodyCD53 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-CD53 (CD53) antibody (PE)CD53 适用: 人 FACS 宿主: 小鼠 Monoclonal 4AC53-2 PE
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- 应用备注
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Positive Control: Daudi, Raji and Jurkat cells. Human leukocytes. Human tonsil and lymph node.
Known Application: Functional Studies (Order Ab without Azide),Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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