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CD40 Ligand 抗体 (AA 108-261)

Name: CD40 Ligand 抗体 (AA 108-261)
Brand: antibodies-online
SKU: ABIN6941189
Availability: OutOfStock

CD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2763 unconjugated

Formalin-fixed, paraffin-embedded human Spleen stained with CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763). (CD40 Ligand 抗体 (AA 108-261))

IHC

SDS-PAGE Analysis Purified CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763). (CD40 Ligand 抗体 (AA 108-261))

SDS

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD40 Mouse Ligand Monoclonal Antibody (CD40LG/2763) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD40 Ligand 抗体 (AA 108-261))

PAr

Flow Cytometric Analysis of Jurkat cells using CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (CD40 Ligand 抗体 (AA 108-261))

FACS

IHC

Immunofluorescence staining of Jurkat cells using CD40 Ligand Mouse Monoclonal Antibody (CD40LG/2763)followed by goat anti-Mouse IgG conjugated to CF488 (green). (CD40 Ligand 抗体 (AA 108-261))

6 images

产品编号 ABIN6941189

发货至: 中国

Datasheet as PDF

抗原 See all CD40 Ligand (CD40LG) 抗体

CD40 Ligand (CD40LG)
抗原表位
16

10

8

6

6

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 108-261
适用
151

81

34

9

8

6

6

2

2

2

2

1

1

1

1

1
人
宿主
97

64

22

15

12

1
小鼠
Compatible Secondaries
克隆类型
111

94

4
单克隆
标记
103

23

21

14

9

3

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD40 Ligand antibody is un-conjugated
应用范围
108

103

54

50

39

31

20

17

14

14

13

13

7

4

4

3

3

3

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

纯化方法

Purified by Protein A/G

免疫原

Recombinant fragment of human CD40LG protein (around aa 108-261) (exact sequence is proprietary)

克隆位点

CD40LG-2763

亚型

IgG1 kappa

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CD40LG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 108-261) antibody
CD40LG 适用: 人 FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal CD40LG-2761 unconjugated

anti-CD40 Ligand (CD40LG) antibody (FITC)
CD40LG 适用: 小鼠 FACS 宿主: Armenian Hamster Monoclonal MR-1 FITC

anti-CD40 Ligand (CD40LG) (AA 79-129) antibody
CD40LG 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 33-62) antibody
CD40LG 适用: 人 WB, FACS, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) (AA 111-261) antibody
CD40LG 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD40 Ligand (CD40LG) antibody (PE)
CD40LG 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 24-31 PE

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG 适用: 人 WB, FACS, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 8H10F5 unconjugated

anti-CD40 Ligand (CD40LG) (AA 47-261) antibody
CD40LG 适用: 人 WB, FACS, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 5A3A9 unconjugated

anti-CD40 Ligand (CD40LG) (AA 47-65), (N-Term) antibody
CD40LG 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Positive Control: Jurkat cells. Human tonsil, spleen or thymus tissue (IHC).
Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

CD40 Ligand (CD40LG)

别名

CD40LG (CD40LG 产品)

别名

CD154 antibody, cd40l antibody, CD40L antibody, HIGM1 antibody, IGM antibody, IMD3 antibody, T-BAM antibody, TNFSF5 antibody, TRAP antibody, gp39 antibody, hCD40L antibody, CD40-L antibody, Cd40l antibody, Ly-62 antibody, Ly62 antibody, Tnfsf5 antibody, CD40 ligand antibody, TNF superfamily member 5 antibody, CD40LG antibody, cd40l antibody, Cd40lg antibody

背景

CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.

分子量

36kDa

基因ID

959

UniProt

P29965

途径

NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints