CD22 抗体 (AA 52-178)
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- 抗原 See all CD22 抗体
- CD22 (CD22 Molecule (CD22))
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抗原表位
- AA 52-178
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD22 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat)
- 特异性
- Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant fragment of human CD22 protein (around aa 52-178) (exact sequence is proprietary)
- 克隆位点
- BLCAM-1796
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CD22 Primary Antibody
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- 应用备注
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Positive Control: Raji or Ramos cells (IF/FACS). Raji cell lysate (WB). Human tonsil, lymph node or spleen (IHC).
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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