CD163 抗体 (AA 43-196)
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- 抗原 See all CD163 抗体
- CD163
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抗原表位
- AA 43-196
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD163 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
- 特异性
- This MAb recognizes a protein of 140 kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression.Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human CD163 fragment (around aa 43-196) (Exact sequence is proprietary)
- 克隆位点
- M130-1210
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CD163 Primary Antibody
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- 应用备注
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Positive Control: HPBL cells. Skin, Spleen, Lung, Placenta or Histiocytoma.
Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- CD163
- 别名
- CD163 (CD163 产品)
- 别名
- DMBT1 antibody, CD163 antibody, CD163v2 antibody, CD163v3 antibody, M130 antibody, MM130 antibody, deleted in malignant brain tumors 1 protein-like antibody, CD163 molecule antibody, CD163 antigen antibody, LOC426820 antibody, CD163 antibody, Cd163 antibody
- 分子量
- 140kDa
- 基因ID
- 9332
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