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CD5 抗体 (AA 269-366)

Name: CD5 抗体 (AA 269-366)
Brand: antibodies-online
SKU: ABIN6941056
Availability: OutOfStock

CD5 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal CD5-2416 unconjugated

Flow Cytometric Analysis of human Jurkat cells using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (CD5 抗体 (AA 269-366))

FACS

Western Blot Analysis of human Jurkat cell lysate using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416). (CD5 抗体 (AA 269-366))

Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse CD5-Monospecific Monoclonal Antibody (CD5/2416) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD5 抗体 (AA 269-366))

PAr

Flow Cytometric Analysis of Ramos cells using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (CD5 抗体 (AA 269-366))

FACS

Formalin-fixed, paraffin-embedded human Tonsil stained with CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416). (CD5 抗体 (AA 269-366))

IHC

Immunofluorescent staining of PFA-fixed Ramos cells using CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416) followed by goat anti-Mouse IgG conjugated to CF488 (green). (CD5 抗体 (AA 269-366))

SDS-PAGE Analysis Purified CD5-Monospecific Mouse Monoclonal Antibody (CD5/2416). (CD5 抗体 (AA 269-366))

SDS

7 images

产品编号 ABIN6941056

发货至: 中国

Datasheet as PDF

抗原 See all CD5 抗体

CD5
抗原表位
16

9

8

5

5

5

5

4

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 269-366
适用
229

80

53

26

22

16

15

12

11

11

11

9

9

2

1
人
宿主
168

82

50

1
小鼠
Compatible Secondaries
克隆类型
225

75
单克隆
标记
101

36

31

27

17

8

7

5

5

4

4

4

4

4

4

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD5 antibody is un-conjugated
应用范围
229

103

69

64

62

50

30

24

19

17

13

13

12

5

3

3

2

2

2

2

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

特异性

Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

纯化方法

Purified by Protein A/G

免疫原

Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)

克隆位点

CD5-2416

亚型

IgG2b kappa

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应用备注

Positive Control: 293T, Ramos or MOLT-4 cells. Tonsil or Lymph Node.
Known Application: Flow Cytometry (1-2 μg/million cells), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

CD5

别名

CD5 (CD5 产品)

别名

CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

分子量

67kDa

基因ID

921

UniProt

P06127