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Recombinant CD3 epsilon 抗体 (AA 23-119)

CD3E 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 兔 Monoclonal C3e-3125R unconjugated Recombinant Antibody
产品编号 ABIN6941035
发货至: 中国
  • 抗原 See all CD3 epsilon (CD3E) 抗体
    CD3 epsilon (CD3E)
    抗体类型
    Recombinant Antibody
    抗原表位
    • 24
    • 19
    • 16
    • 16
    • 13
    • 12
    • 9
    • 8
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 23-119
    适用
    • 215
    • 154
    • 51
    • 42
    • 18
    • 8
    • 8
    • 8
    • 7
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    宿主
    • 132
    • 112
    • 33
    • 23
    • 20
    • 8
    • 1
    • 1
    • 1
    克隆类型
    • 210
    • 115
    • 6
    单克隆
    标记
    • 121
    • 31
    • 28
    • 20
    • 15
    • 8
    • 8
    • 6
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD3 epsilon antibody is un-conjugated
    应用范围
    • 237
    • 134
    • 94
    • 80
    • 70
    • 69
    • 27
    • 27
    • 24
    • 22
    • 14
    • 13
    • 13
    • 12
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)
    特异性
    Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant human CD3e fragment (around aa 23-119) (exact sequence is proprietary)
    克隆位点
    C3e-3125R
    亚型
    IgG
    Top Product
    Discover our top product CD3E Primary Antibody
  • 应用备注

    Positive Control: Jurkat and MOLT-4 cells. Tonsil or lymph node.

    Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    CD3 epsilon (CD3E)
    别名
    CD3E (CD3E 产品)
    别名
    cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
    分子量
    20-25kDa
    基因ID
    916
    UniProt
    P07766
    途径
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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