CD3 epsilon 抗体
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- 抗原 See all CD3 epsilon (CD3E) 抗体
- CD3 epsilon (CD3E)
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适用
- 人, 猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD3 epsilon antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Activation (Act), Proliferation Assay (ProA)
- 特异性
- Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
- 免疫原
- Stimulated human leukocytes
- 克隆位点
- CRIS-7
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CD3E Primary Antibody
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- 应用备注
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Positive Control: Jurkat cells. Tonsil and lymph node.
Known Application: Induce T cell activation and proliferation (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 分子量
- 20kDa
- 基因ID
- 916
- UniProt
- P07766
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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