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Cyclin E1 抗体 (AA 10-176)

CCNE1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal CCNE1-2460 unconjugated
产品编号 ABIN6940994
发货至: 中国
  • 抗原 See all Cyclin E1 (CCNE1) 抗体
    Cyclin E1 (CCNE1)
    抗原表位
    • 27
    • 16
    • 16
    • 13
    • 8
    • 8
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 10-176
    适用
    • 144
    • 37
    • 33
    • 3
    • 2
    • 2
    • 2
    宿主
    • 121
    • 22
    • 1
    小鼠
    克隆类型
    • 115
    • 29
    单克隆
    标记
    • 79
    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This Cyclin E1 antibody is un-conjugated
    应用范围
    • 99
    • 47
    • 46
    • 42
    • 32
    • 28
    • 20
    • 13
    • 12
    • 8
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)
    克隆位点
    CCNE1-2460
    亚型
    IgG2b kappa
  • 应用备注

    Positive Control: Jurkat, MCF, A431, HeLa cells. Breast or Colon Carcinoma.

    Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    Cyclin E1 (CCNE1)
    别名
    CCNE1 (CCNE1 产品)
    别名
    CCNE1 antibody, ccne antibody, cyclinE antibody, CCNE antibody, AW538188 antibody, CycE1 antibody, CYCLE antibody, Ccne antibody, cyce2 antibody, cycE3 antibody, cyce antibody, fa18e07 antibody, wu:fa18e07 antibody, cyclin E1 antibody, cyclin E1 L homeolog antibody, cyclin E1 S homeolog antibody, CCNE1 antibody, ccne1 antibody, Ccne1 antibody, ccne1.L antibody, ccne1.S antibody
    背景
    Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
    分子量
    53kDa
    基因ID
    898
    UniProt
    P24864
    途径
    Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
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