Cyclin A 抗体
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- 抗原 See all Cyclin A (CCNA2) 抗体
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
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适用
- 人, 小鼠, Cow, Green Monkey, Mink
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Cyclin A antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- It recognizes a protein of 54 kDa, which is identified as cyclin A. Its epitope is located amino acids 144-148 of human Cyclin A2. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Full length bovine Cyclin A protein
- 克隆位点
- E67
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CCNA2 Primary Antibody
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anti-Cyclin A2 (CCNA2) antibody
KO Validated CCNA2 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 105-233) antibodyCCNA2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6B4D11 unconjugated
anti-Cyclin A2 (CCNA2) (AA 190-270) antibodyCCNA2 适用: 人, 小鼠, 大鼠, 猴 WB, IF, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 1-200) antibodyCCNA2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 51-84) antibodyCCNA2 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 375-431) antibodyCCNA2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) antibodyCCNA2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (C-Term) antibodyCCNA2 适用: 人, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) antibodyCCNA2 适用: 人 ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin A2 (CCNA2) (AA 51-84), (N-Term) antibodyCCNA2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB14617 unconjugated
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- 应用备注
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Positive Control: K-562 and HeLa cells. Human tonsil or lymph node.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Cyclin A (CCNA2) (Cyclin A2 (CCNA2))
- 别名
- CCNA2 (CCNA2 产品)
- 别名
- CCN1 antibody, CCNA antibody, AA408589 antibody, Ccn-1 antibody, Ccn1 antibody, Ccna antibody, CycA2 antibody, Cyca antibody, cb671 antibody, chunp6924 antibody, wu:fi36f03 antibody, CYCA antibody, Cyclin-A2 antibody, ccna2 antibody, ccn1 antibody, ccna antibody, ccna1 antibody, Cyclin-A antibody, cyclinA antibody, cyclin A2 antibody, cyclin A2 S homeolog antibody, cyclin A2 L homeolog antibody, CCNA2 antibody, Ccna2 antibody, ccna2 antibody, ccna2.S antibody, ccna2.L antibody
- 分子量
- 54kDa
- 基因ID
- 890
- UniProt
- P20248
- 途径
- PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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