MAML2 抗体
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- 抗原 See all MAML2 抗体
- MAML2 (Mastermind-Like 2 (MAML2))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MAML2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human MAML2 fragment
- 克隆位点
- MAML2-1302
- 亚型
- IgG2a kappa
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anti-Mastermind-Like 2 (MAML2) (AA 347-506) antibody
MAML2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 796-894) antibodyMAML2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A1 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 780-814) antibodyMAML2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB52651 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 750-850) antibodyMAML2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 169-218) antibodyMAML2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 324-373) antibodyMAML2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- MAML2 (Mastermind-Like 2 (MAML2))
- 别名
- MAML2 (MAML2 产品)
- 别名
- 5930431H10 antibody, BC032967 antibody, MAM-3 antibody, MAM2 antibody, MAM3 antibody, MLL-MAML2 antibody, mastermind like 2 (Drosophila) antibody, mastermind-like transcriptional coactivator 2 antibody, mastermind like transcriptional coactivator 2 antibody, Maml2 antibody, MAML2 antibody
- 背景
- MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.
- 分子量
- 125kDa
- 基因ID
- 84441
- UniProt
- Q8IZL2
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