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Vimentin 抗体 (AA 2-466)

Name: Vimentin 抗体 (AA 2-466)
Brand: antibodies-online
SKU: ABIN6940884
Availability: OutOfStock

VIM 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal VIM-3736 unconjugated

Western Blot Analysis of U87 cell lysate using Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

Analysis of Protein Array containing more than 21,000 full-length human proteins using Vimentin Mouse Monoclonal Antibody (VIM/3736) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (Vimentin 抗体 (AA 2-466))

PAr

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

IHC

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

IHC

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VIM/3736). (Vimentin 抗体 (AA 2-466))

5 images

产品编号 ABIN6940884

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

18

16

15

15

15

11

9

7

7

4

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466
适用
341

133

130

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
人
宿主
195

129

11

6

6

5

2
小鼠
Compatible Secondaries
克隆类型
211

142
单克隆
标记
203

19

14

14

10

7

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
286

130

105

100

96

64

46

40

39

33

23

19

8

5

4

3

3

2

2

2

1

1

1

1

1

1

1

1
Immunohistochemistry (IHC), Staining Methods (StM)

特异性

This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

无交叉反应

小鼠, 大鼠

纯化方法

Purified by Protein A/G

免疫原

A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

克隆位点

VIM-3736

亚型

IgG2b kappa

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anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

Positive Control: U87, A375, Raji, HEP2 cells. Sarcomas or Melanomas.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

Vimentin (VIM)

别名

VIM (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

分子量

57-60kDa

基因ID

7431

UniProt

P08670

途径

Caspase Cascade in Apoptosis