Villin 1 抗体 (AA 179-311)
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- 抗原 See all Villin 1 (VIL1) 抗体
- Villin 1 (VIL1)
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抗原表位
- AA 179-311
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Villin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary)
- 克隆位点
- VIL1-1314
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product VIL1 Primary Antibody
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- 应用备注
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Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.
Known Application: Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Villin 1 (VIL1)
- 别名
- VIL1 (VIL1 产品)
- 别名
- D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody
- 分子量
- 93kDa
- 基因ID
- 7429
- UniProt
- P09327
- 途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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