我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

TYMS 抗体 (AA 60-174)

Name: TYMS 抗体 (AA 60-174)
Brand: antibodies-online
SKU: ABIN6940822
Availability: OutOfStock

TYMS 适用: 人 WB, IF, FACS, IHC, ISt, RIA, StM 宿主: 小鼠 Monoclonal TYMS-1884 unconjugated

Western Blot Analysis of human HeLa cell lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884). (TYMS 抗体 (AA 60-174))

Analysis of Protein Array containing more than 19,000 full-length human proteins using Thymidylate Synthase Monospecific Mouse Monoclonal Antibody (TYMS/1884) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (TYMS 抗体 (AA 60-174))

PAr

Flow Cytometric Analysis of PFA-fixed MOLT4 cells. (TYMS 抗体 (AA 60-174))

FACS

Flow Cytometric Analysis of PFA-fixed Ramos cells. (TYMS 抗体 (AA 60-174))

FACS

Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884). (TYMS 抗体 (AA 60-174))

IHC

Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU. (TYMS 抗体 (AA 60-174))

SDS-PAGE Analysis Purified Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884). (TYMS 抗体 (AA 60-174))

SDS

7 images

产品编号 ABIN6940822

发货至: 中国

Datasheet as PDF

抗原 See all TYMS 抗体

TYMS (Thymidylate Synthetase (TYMS))
抗原表位
15

12

5

5

4

3

3

2

1

1

1

1
AA 60-174
适用
97

29

14

3

2

2

2

1

1

1
人
宿主
55

41

1
小鼠
Compatible Secondaries
克隆类型
50

47
单克隆
标记
45

7

4

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TYMS antibody is un-conjugated
应用范围
50

41

33

22

19

18

17

17

14

13

9

6

6

3

3

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Radioimmunoassay (RIA), Staining Methods (StM)

特异性

It recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

纯化方法

Purified by Protein A/G

免疫原

Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)

克隆位点

TYMS-1884

亚型

IgG2c kappa

Top Product

Discover our top product TYMS Primary Antibody
anti-Thymidylate Synthetase (TYMS) (AA 36-313) antibody
TYMS 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibody
TYMS 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3A1 unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D6 unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5H8 unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibody
TYMS 适用: 人 WB, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 2B2 unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 265-294), (C-Term) antibody
TYMS 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB20041 unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 2-54) antibody
TYMS 适用: 人 WB, IF, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 60-174) antibody
TYMS 适用: 人 IF, FACS, ELISA, RIA, Coat 宿主: 小鼠 Monoclonal rTYMS-1884 unconjugated Recombinant Antibody

应用备注

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

有效期

24 months
抗原

TYMS (Thymidylate Synthetase (TYMS))

别名

TYMS (TYMS 产品)

别名

FN1 antibody, ECK2823 antibody, JW2795 antibody, ts antibody, Ts antibody, HST422 antibody, TMS antibody, TS antibody, thymidylate synthetase antibody, contains intron in T4 phage antibody, ThyE antibody, thymidylate synthase antibody, thymidylate synthetase L homeolog antibody, TYMS antibody, thyA antibody, td antibody, thyE antibody, DDA3937_RS04960 antibody, tyms.L antibody, XBJ1_RS15770 antibody, EAMY_RS20680 antibody, Tyms antibody

物质类

Viral Protein

分子量

36kDa

基因ID

7298

UniProt

P04818

途径

Mitotic G1-G1/S Phases