Topoisomerase II alpha 抗体 (AA 1352-1493)
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- 抗原 See all Topoisomerase II alpha (TOP2A) 抗体
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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抗原表位
- AA 1352-1493
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Topoisomerase II alpha antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
- 特异性
- It recognizes a 170 kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human Topoisomerase II alpha fragment (aa1352-1493)
- 克隆位点
- TOP2A-1361
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product TOP2A Primary Antibody
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- 应用备注
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Positive Control: HeLa cells. Tonsil.
Known Application: Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- 别名
- TOP2A (TOP2A 产品)
- 别名
- TOP2 antibody, TP2A antibody, TOP2A antibody, Top-2 antibody, TOP-2 antibody, F12B17.110 antibody, F12B17_110 antibody, thimet metalloendopeptidase 2 antibody, fc08c07 antibody, wu:fc08c07 antibody, DNA topoisomerase II alpha antibody, DNA topoisomerase II, alpha isozyme antibody, topoisomerase (DNA) II alpha antibody, Zincin-like metalloproteases family protein antibody, TOP2A antibody, Bm1_08705 antibody, Top2a antibody, AT5G10540 antibody, top2a antibody
- 分子量
- 170kDa
- 基因ID
- 7153
- UniProt
- P11388
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
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