TGFA 抗体 (C-Term)
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- 抗原 See all TGFA 抗体
- TGFA (Transforming Growth Factor, alpha (TGFA))
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抗原表位
- C-Term
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适用
- 人, 大鼠, 小鼠, Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TGFA antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Staining Methods (StM), Coating (Coat)
- 特异性
- This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)
- 克隆位点
- MF9
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TGFA Primary Antibody
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- 应用备注
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Positive Control: Jurkat or Ramos cells. Testicular Carcinoma.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- TGFA (Transforming Growth Factor, alpha (TGFA))
- 别名
- TGFA (TGFA 产品)
- 别名
- TFGA antibody, LOC100009482 antibody, TGF alpha antibody, TGFA antibody, wa-1 antibody, wa1 antibody, RATTGFAA antibody, TGFAA antibody, NV15606 antibody, si:ch211-10e8.7 antibody, transforming growth factor alpha antibody, transforming growth factor alpha L homeolog antibody, transforming growth factor-alpha antibody, transforming growth factor, alpha antibody, TGFA antibody, TGF-A antibody, Tgfa antibody, tgfa.L antibody, LOC100121300 antibody, LOC100009150 antibody, tgfa antibody
- 分子量
- ~6kDa
- 基因ID
- 7039
- UniProt
- P01135
- 途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
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