Transferrin 抗体 (AA 311-445)
-
- 抗原 See all Transferrin (TF) 抗体
- Transferrin (TF)
-
抗原表位
- AA 311-445
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Transferrin antibody is un-conjugated
-
应用范围
- ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant fragment (around aa 311-445) of human TF protein (exact sequence is proprietary)
- 克隆位点
- TF-3001
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TF Primary Antibody
-
-
- 应用备注
-
Positive Control: Jurkat, HeLa, MCF-7 or K562 cells. Human liver.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
- 抗原
- Transferrin (TF)
- 别名
- Transferrin (Early Marker of Oligodendrocytes) (TF 产品)
- 背景
- Iron (Fe) is a tightly metabolically controlled mineral and growth factor present in all living cells. Iron not bound in erythrocyte hemoglobin is transported by transferrin (Tf), the iron transport protein of vertebrate serum. The transferrin protein contains two homologous domains, each of which contain an Fe-binding site. The majority of transferrin is synthesized in the liver and secreted into the blood, but it is also produced in lower amounts in testis and brain as well as in oligodendrocytes, where transferrin is an early marker of oligodendrocyte differentiation. From the blood, transferrin is internalized by erythroblasts and reticulocytes upon binding the transferrin receptor (TfR), also designated CD71, through a system of coated pits and vesicles. After Fe release, transferrin is returned to the extracellular medium, where it can be reused. Defects in the transferrin gene results in atransferrinemia, a rare autosomal recessive disorder characterized by microcytic anemia and iron loading.
- 分子量
- 79kDa
- 基因ID
- 7018
- UniProt
- P02787
- 途径
- Transition Metal Ion Homeostasis
-