VISTA 抗体
-
- 抗原 See all VISTA 抗体
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This VISTA antibody is un-conjugated
-
应用范围
- ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant full-length human VISTA protein
- 克隆位点
- VISTA-3007
- 亚型
- IgG2b kappa
-
-
- 应用备注
-
Positive Control: Spleen or Placenta.
Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
- 抗原
- VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))
- 别名
- VSIR (VISTA 产品)
- 别名
- MGC112715 antibody, MGC151567 antibody, B7-H5 antibody, B7H5 antibody, GI24 antibody, PP2135 antibody, SISP1 antibody, Dies1 antibody, VISTA antibody, V-set immunoregulatory receptor antibody, vsir antibody, Vsir antibody, VSIR antibody
- 背景
- VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
- 分子量
- 34kDa
- 基因ID
- 64115
- UniProt
- Q9H7M9
- 途径
- Cancer Immune Checkpoints
-