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POU2AF1 抗体 (AA 148-255)

POU2AF1 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal BOB1-2424 unconjugated
产品编号 ABIN6940356
发货至: 中国
  • 抗原 See all POU2AF1 抗体
    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
    抗原表位
    • 15
    • 15
    • 14
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    AA 148-255
    适用
    • 61
    • 20
    • 6
    宿主
    • 68
    • 8
    • 2
    小鼠
    克隆类型
    • 68
    • 10
    单克隆
    标记
    • 30
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POU2AF1 antibody is un-conjugated
    应用范围
    • 48
    • 30
    • 26
    • 26
    • 21
    • 18
    • 14
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
    克隆位点
    BOB1-2424
    亚型
    IgG2b kappa
  • 应用备注

    Positive Control: Raji, Ramos cells. Spleen or Tonsil.

    Known Application: Flow Cytometry (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
    别名
    POU2AF1 (POU2AF1 产品)
    别名
    BOB1 antibody, OBF-1 antibody, OBF1 antibody, OCAB antibody, BOB-1 antibody, BOB.1 antibody, Bob-1 antibody, Bob1 antibody, OBF.1 antibody, OCA-B antibody, POU class 2 associating factor 1 antibody, POU domain, class 2, associating factor 1 antibody, POU2AF1 antibody, Pou2af1 antibody
    背景
    BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
    分子量
    35kDa
    基因ID
    5450
    UniProt
    Q16633
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