POU2AF1 抗体 (AA 148-255)
-
- 抗原 See all POU2AF1 抗体
- POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
-
抗原表位
- AA 148-255
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This POU2AF1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant fragment (around aa 148-255) of human BOB1 (POU2AF1) protein (exact sequence is proprietary)
- 克隆位点
- BOB1-2424
- 亚型
- IgG2b kappa
-
-
- 应用备注
-
Positive Control: Raji, Ramos cells. Spleen or Tonsil.
Known Application: Flow Cytometry (1-2 μg/mL), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
-
- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
-
- 抗原
- POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))
- 别名
- POU2AF1 (POU2AF1 产品)
- 别名
- BOB1 antibody, OBF-1 antibody, OBF1 antibody, OCAB antibody, BOB-1 antibody, BOB.1 antibody, Bob-1 antibody, Bob1 antibody, OBF.1 antibody, OCA-B antibody, POU class 2 associating factor 1 antibody, POU domain, class 2, associating factor 1 antibody, POU2AF1 antibody, Pou2af1 antibody
- 背景
- BOB.1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB.1. Conversely, only some cases of classical Hodgkin lymphoma show BOB.1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells.Expression of BOB.1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia.B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
- 分子量
- 35kDa
- 基因ID
- 5450
- UniProt
- Q16633
-