Prohibitin 抗体 (AA 167-261)
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- 抗原 See all Prohibitin (PHB) 抗体
- Prohibitin (PHB)
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抗原表位
- AA 167-261
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Prohibitin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)
- 克隆位点
- PHB-3230
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product PHB Primary Antibody
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- 应用备注
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Positive Control: Liver, Kidney, Heart or Endometrium (IHC).
Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Prohibitin (PHB)
- 别名
- PHB (PHB 产品)
- 别名
- phb1 antibody, MGC75944 antibody, PHB antibody, Phb antibody, ACYPI000080 antibody, phb antibody, PHB1 antibody, Bap32 antibody, sr:nyz128 antibody, wu:fa18f07 antibody, wu:fb09f05 antibody, prohibitin1 antibody, xphb1 antibody, prohibitin antibody, prohibitin-2 antibody, prohibitin L homeolog antibody, PHB antibody, phb antibody, LOC484431 antibody, LOC5576054 antibody, Phb antibody, phb.L antibody
- 分子量
- 30kDa
- 基因ID
- 5245
- UniProt
- P35232
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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