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- 抗原 See all Geminin (GMNN) 抗体
- Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Geminin antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human full-length protein
- 克隆位点
- CPTC-GMMN-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product GMNN Primary Antibody
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anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 110-209) antibody
GMNN 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A8 unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 1-209) antibodyGMNN 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 1-206) antibodyGMNN 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 50-100) antibodyGMNN 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 110-142) antibodyGMNN 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 1-209) antibodyGMNN 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 110-209) antibodyGMNN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) (AA 1-209) antibodyGMNN 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Geminin, DNA Replication Inhibitor (GMNN) antibodyGMNN 适用: 人 ELISA, IF 宿主: 兔 Monoclonal 8H8 unconjugated Recombinant Antibody
anti-Geminin, DNA Replication Inhibitor (GMNN) antibodyGMNN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H7 unconjugated
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- 应用备注
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Positive Control: HEK293, HeLa, MCF-7, Tonsil, Triple-negative breast cancer.
Known Application: Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))
- 别名
- GMMN (GMNN 产品)
- 别名
- CG3183 antibody, Dm Geminin antibody, Dm geminin antibody, Dmel\\CG3183 antibody, Gem antibody, Geminin antibody, anon-WO0257455.1 antibody, gem antibody, l(2)k14019 antibody, v(2)k09107 antibody, sb:cb254 antibody, zgc:66217 antibody, GB16871 antibody, GMNN antibody, geminin antibody, dyak_GLEANR_833 antibody, DyakGE24654 antibody, GE24654 antibody, LOC100049517 antibody, AW546347 antibody, geminin, DNA replication inhibitor antibody, CG3183 gene product from transcript CG3183-RA antibody, geminin antibody, GE24654 gene product from transcript GE24654-RA antibody, GMNN antibody, geminin antibody, gmnn antibody, LOC410093 antibody, Dyak\geminin antibody, Gmnn antibody
- 背景
- Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphasepromoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitinmediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.
- 分子量
- 35kDa
- 基因ID
- 51053
- UniProt
- O75496
- 途径
- EGFR Signaling Pathway, DNA Replication
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