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CD73 抗体

NT5E 适用: 人 WB, FACS, IHC, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated

Western Blot Analysis of A431 and PC-3 cell lysate using CD73 Mouse Monoclonal Antibody (NT5E/2545). (CD73 抗体)

Analysis of Protein Array containing more than 19,000 full-length human proteins using CD73 Mouse Recombinant Monoclonal Antibody (NT5E/2545) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (CD73 抗体)

Flow Cytometric Analysis of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2545) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (CD73 抗体)

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2545). (CD73 抗体)

Formalin-fixed, paraffin-embedded human Pancreatic Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2545). (CD73 抗体)

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2545). (CD73 抗体)

Formalin-fixed, paraffin-embedded human Tonsil stained with CD73 Mouse Monoclonal Antibody (NT5E/2545). (CD73 抗体)

Formalin-fixed, paraffin-embedded human Tonsil stained with CD73 Mouse Monoclonal Antibody (NT5E/2545). (CD73 抗体)

Immunofluorescence staining of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2545) followed by goat anti-Mouse IgG conjugated to CF488 (green). (CD73 抗体)

SDS-PAGE Analysis Purified CD73 Mouse Monoclonal Antibody (NT5E/2545). (CD73 抗体)

10 images

产品编号 ABIN6940229

发货至: 中国

Datasheet as PDF

抗原 See all CD73 (NT5E) 抗体

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
适用
107

60

30

18

18

16

4

2

1

1

1

1

1
人
宿主
88

29

9

2
小鼠
Compatible Secondaries
克隆类型
79

49
单克隆
标记
53

12

11

7

7

4

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD73 antibody is un-conjugated
应用范围
79

50

43

37

15

14

14

12

8

7

5

3

3

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Staining Methods (StM), Coating (Coat)

纯化方法

Purified by Protein A/G

免疫原

Recombinant full length human NT5E protein

克隆位点

NT5E-2545

亚型

IgG1 kappa

Top Product

Discover our top product NT5E Primary Antibody
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody
NT5E 适用: 人 WB, FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibody
NT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibody
NT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)
NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibody
NT5E 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibody
NT5E 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibody
NT5E 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated

应用备注

Positive Control: Tonsil, Prostate or Lung Carcinoma.
Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

别名

NT5E (NT5E 产品)

别名

CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

背景

CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.

分子量

71kDa

基因ID

4907

UniProt

P21589

途径

Synaptic Membrane, Ribonucleoside Biosynthetic Process

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