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- 抗原 See all MITF 抗体
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
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适用
- 人, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MITF antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
- 无交叉反应
- 小鼠, 大鼠
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant full-length human MiTF protein
- 克隆位点
- MITF-915
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MITF Primary Antibody
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anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 351-450) antibody
MITF 适用: 小鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer180), (pSer73) antibodyMITF 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-28), (N-Term) antibodyMITF 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB18810 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-114) antibodyMITF 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3A2E2 unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人, 犬 IHC, IF, FACS, StM 宿主: 兔 Monoclonal MITF-2987R unconjugated Recombinant Antibody
anti-Microphthalmia-Associated Transcription Factor (MITF) (AA 1-280) antibodyMITF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) (Ser180), (Ser73) antibodyMITF 适用: 人, 小鼠 WB, IHC, ELISA, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Microphthalmia-Associated Transcription Factor (MITF) antibodyMITF 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal D5 unconjugated
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- 应用备注
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Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.
Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- MITF (Microphthalmia-Associated Transcription Factor (MITF))
- 别名
- MITF (MITF 产品)
- 别名
- MITF antibody, ws2a antibody, mitfa antibody, MITF-A antibody, LOC100313655 antibody, mitfb antibody, CMM8 antibody, MI antibody, WS2 antibody, WS2A antibody, bHLHe32 antibody, BCC2 antibody, Bhlhe32 antibody, Gsfbcc2 antibody, Vitiligo antibody, Wh antibody, bw antibody, mi antibody, vit antibody, MITF-H antibody, MITF-M antibody, CMI9 antibody, melanogenesis associated transcription factor antibody, microphthalmia-associated transcription factor antibody, microphthalmia-associated transcription factor S homeolog antibody, Microphthalmia antibody, MITF antibody, mitf antibody, LOC100313655 antibody, mitf.S antibody, LOC100534525 antibody, MICROP antibody, Mitf antibody
- 分子量
- 52-56kDa (doublet)
- 基因ID
- 4286
- UniProt
- O75030
- 途径
- Chromatin Binding
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