MCM7 抗体 (AA 195-319)
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- 抗原 See all MCM7 抗体
- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
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抗原表位
- AA 195-319
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MCM7 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human MCM7 protein fragment (aa195-319) (exact sequence is proprietary)
- 克隆位点
- MCM7-1467
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product MCM7 Primary Antibody
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- 应用备注
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Positive Control: MCF-7, HEK293, A549, Raji or Ramos cells. Tonsil.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
- 别名
- MCM7 (MCM7 产品)
- 背景
- The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.
- 分子量
- 88kDa
- 基因ID
- 4176
- UniProt
- P33993
- 途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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