Recombinant EpCAM 抗体 (Extracellular Domain)
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- 抗原 See all EpCAM (EPCAM) 抗体
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
- 抗体类型
- Recombinant Antibody
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抗原表位
- AA 100-224, Extracellular Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This EpCAM antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
- 免疫原
- Recombinant human EpCAM fragment from extracellular domain (around aa100-224) (exact sequence is proprietary)
- 克隆位点
- EGP40-2571R
- 亚型
- IgG
- Top Product
- Discover our top product EPCAM Primary Antibody
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- 应用备注
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Positive Control: MCF-7 or HT29 cells. Breast tumor.
Known Application: Flow Cytometry (1-2 μg/million cells). Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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