EpCAM 抗体 (N-Term)
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- 抗原 See all EpCAM (EPCAM) 抗体
- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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抗原表位
- AA 20-60, N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This EpCAM antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- Recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
- 无交叉反应
- 小鼠, 大鼠
- 纯化方法
- Purified by Protein A/G
- 免疫原
- A synthetic peptide (around aa 20-60) from the N-terminus of human TACSTD1 protein
- 克隆位点
- EGP40-826
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product EPCAM Primary Antibody
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibodyEPCAM 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibodyEPCAM 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rEGP40-1372 unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 FACS, IF, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1373 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibodyEPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1372 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibodyEPCAM 适用: 人 FACS, IHC, StM 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibodyEPCAM 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibodyEPCAM 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7B5C10 unconjugated
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- 应用备注
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Positive Control: HT29 or SK-OV-3 cells (FACS/IF). Breast carcinoma (IHC).
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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