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EpCAM 抗体 (Extracellular Domain)

Name: EpCAM 抗体 (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN6939977
Availability: OutOfStock

EPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1372 unconjugated

Formalin-fixed, paraffin-embedded human Colorectal Carcinoma stained with EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM 抗体 (Extracellular Domain))

IHC

Western Blot of human lung lysate using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM 抗体 (Extracellular Domain))

Analysis of Protein Array containing >19,000 full-length human proteins using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (EpCAM 抗体 (Extracellular Domain))

PAr

Confocal Immunofluorescence of human Colo-rectal Carcinoma. (EpCAM 抗体 (Extracellular Domain))

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red). (EpCAM 抗体 (Extracellular Domain))

FACS

Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM 抗体 (Extracellular Domain))

IHC

Immunofluorescent staining of MCF-7 cells. (EpCAM 抗体 (Extracellular Domain))

SDS-PAGE Analysis Purified EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM 抗体 (Extracellular Domain))

SDS

Western Blot of 293, A431 and HCT116 cell lysate using EpCAM-Monospecific Mouse Monoclonal Antibody (EGP40/1372). (EpCAM 抗体 (Extracellular Domain))

9 images

产品编号 ABIN6939977

发货至: 中国

Datasheet as PDF

抗原 See all EpCAM (EPCAM) 抗体

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
抗原表位
56

41

33

27

16

16

15

14

13

11

10

8

5

5

4

4

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 77-202, Extracellular Domain
适用
293

79

39

2

1

1
人
宿主
152

151

11

1
小鼠
Compatible Secondaries
克隆类型
188

123

2
单克隆
标记
152

20

18

13

9

9

8

8

8

8

8

7

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This EpCAM antibody is un-conjugated
应用范围
218

178

120

110

90

33

26

24

24

19

18

16

15

12

3

2

2

2

2

1

1
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

纯化方法

Purified by Protein A/G

免疫原

Recombinant human EpCAM fragment from extracellular domain (around aa 77-202) (exact sequence is proprietary)

克隆位点

EGP40-1372

亚型

IgG1 kappa

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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibody
EPCAM 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibody
EPCAM 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 20-60), (N-Term) antibody
EPCAM 适用: 人 FACS, IHC, StM 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal rEGP40-1372 unconjugated Recombinant Antibody

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM 适用: 人 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal EGP40-1373 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 77-202), (Extracellular Domain) antibody
EPCAM 适用: 人 FACS, IF, IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 280-350), (Cytoplasmic Domain) antibody
EPCAM 适用: 人, 小鼠, 大鼠 IHC, StM 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibody
EPCAM 适用: 人 WB, FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7B5C10 unconjugated

anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 221-314) antibody
EPCAM 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

应用备注

Positive Control: MCF-7, HT29 cells. Ovarian carcinoma.
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

10 mM PBS with 0.05 % BSA & 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

有效期

24 months
抗原

EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

别名

TACSTD1 (EPCAM 产品)

别名

DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody

背景

EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

分子量

40-43kDa

基因ID

4072

UniProt

P16422