IgL 抗体
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- 抗原 See all IgL products
- IgL
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IgL antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Staining Methods (StM)
- 特异性
- This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Purified IgG from human serum
- 克隆位点
- N10-2
- 亚型
- IgG1 kappa
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- 应用备注
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Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
Known Application: Flow Cytometry (0.5-1 μg/million cells in 0.1ml), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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