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HSPD1 抗体

HSPD1 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Hamster, 犬, Cow, 兔, Pig, 绵羊, Drosophila melanogaster, 非洲爪蟾 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal LK1 unconjugated
产品编号 ABIN6939665
发货至: 中国
  • 抗原 See all HSPD1 抗体
    HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
    适用
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    人, 小鼠, 大鼠, 小鸡, 猴, Hamster, 犬, Cow, 兔, Pig, 绵羊, Drosophila melanogaster, 非洲爪蟾
    宿主
    • 120
    • 114
    • 3
    小鼠
    克隆类型
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    单克隆
    标记
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    This HSPD1 antibody is un-conjugated
    应用范围
    • 186
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)
    特异性
    Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). Hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.
    无交叉反应
    Bacteria, Nematode, Spinach (Spinacia oleracea)
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant human HSP60 protein
    克隆位点
    LK1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product HSPD1 Primary Antibody
  • 应用备注

    Positive Control: MCF-7 or HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (0.25-0.5 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
    别名
    HSPD1 (HSPD1 产品)
    分子量
    60kDa
    基因ID
    3329
    UniProt
    P10809
    途径
    Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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