HSP27 抗体
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- 抗原 See all HSP27 (HSPB1) 抗体
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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适用
- 人, 大鼠, 小鼠, 绵羊, 猴, 小鸡, Chimpanzee
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HSP27 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)
- 特异性
- MAb G3.1 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50 % homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Partially Purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
- 克隆位点
- G3-1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product HSPB1 Primary Antibody
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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, FACS, IP 宿主: 兔 Monoclonal 10C11 unconjugated Recombinant Antibody
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibodyHSPB1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibodyHSPB1 适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 小鼠 Monoclonal 5D12-A12 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 HRP
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (FITC)HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 FITC
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- 应用备注
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Positive Control: MCF-7 cells. ~50 % of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR. HeLa cells.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.25-0.5 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- 别名
- HSPB1 (HSPB1 产品)
- 别名
- CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody
- 分子量
- 27kDa
- 基因ID
- 3315
- UniProt
- P04792
- 途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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