ICOSLG 抗体 (AA 23-149)
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- 抗原 See all ICOSLG 抗体
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
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抗原表位
- AA 23-149
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ICOSLG antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)
- 特异性
- This MAb recognizes a protein of 36 kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149) (exact sequence is proprietary)
- 克隆位点
- ICOSL-3111
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product ICOSLG Primary Antibody
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- 应用备注
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Positive Control: U937, HL60, HuT78 and HUVEC lysates (WB). Human tonsil tissue (IHC).
Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))
- 别名
- ICOSLG (ICOSLG 产品)
- 别名
- ICOSLG antibody, AU044799 antibody, B7-H2 antibody, B7RP-1 antibody, B7h antibody, BG071784 antibody, GI50 antibody, GL50 antibody, GL50-B antibody, ICOS-L antibody, Icoslg antibody, LICOS antibody, Ly115l antibody, mKIAA0653 antibody, B7H2 antibody, B7RP1 antibody, CD275 antibody, ICOSL antibody, Icosl antibody, RGD1562791 antibody, inducible T-cell costimulator ligand antibody, icos ligand antibody, inducible T-cell co-stimulator ligand antibody, ICOSLG antibody, Icosl antibody, Icoslg antibody
- 分子量
- 36kDa
- 基因ID
- 23308
- UniProt
- O75144
- 途径
- Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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