ENO2/NSE 抗体 (AA 416-433)
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- 抗原 See all ENO2/NSE (ENO2) 抗体
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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抗原表位
- AA 416-433
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ENO2/NSE antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- Recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
- 克隆位点
- ENO2-1375
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product ENO2 Primary Antibody
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- 应用备注
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Positive Control: HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
Known Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.2-0.4 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- 别名
- ENO2 (ENO2 产品)
- 别名
- ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody
- 分子量
- ~50kDa
- 基因ID
- 2026
- UniProt
- P09104
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