Emerin 抗体 (AA 56-167)
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- 抗原 See all Emerin (EMD) 抗体
- Emerin (EMD)
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抗原表位
- AA 56-167
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Emerin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human Emerin protein fragment (around aa 56-167) (exact sequence is proprietary)
- 克隆位点
- EMD-2167
- 亚型
- IgG1 kappa
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- 应用备注
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Positive Control: K-562, HeLa or Jurkat cells. Kidney or Skin.
Known Application: Western Blot (0.5-2 μg/mL), Immunofluorescence (1-3 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- Emerin (EMD)
- 别名
- EMD (EMD 产品)
- 背景
- Emerin is a member of the nuclear lamina associated protein family. It is ubiquitously expressed and localized to the nuclear membrane in normal cells. Mutations of the gene that encodes emerin result in the X-linked recessive disease Emery-Dreifuss muscular dystrophy (EDMD), which is characterized by slowly progressing contractures, skeletal muscle wasting and cardiomyopathy. Reportedly, lack of Emerin expression is one cause of EDMD. Emerin is involved in the association of the nuclear membrane with the lamina, and is localized specifically to desmosomes and fasciae adherents in the heart. Identification of nuclear membrane irregularities with anti-emerin antibody has been reported useful in diagnosing papillary thyroid carcinoma.
- 分子量
- 37kDa
- 基因ID
- 2010
- UniProt
- P50402
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