Desmoglein 3 抗体
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- 抗原 See all Desmoglein 3 (DSG3) 抗体
- Desmoglein 3 (DSG3)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Desmoglein 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
- 特异性
- Recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant human DSG3 extracellular protein fragment
- 克隆位点
- 5G11
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product DSG3 Primary Antibody
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- 应用备注
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Positive Control: A431 cells. Lung squamous cell carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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