CLTA/CLTB 抗体 (N-Term)
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- 抗原 See all CLTA/CLTB 抗体
- CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CLTA/CLTB antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
- 特异性
- Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Purified recombinant N-terminal fragment of human Clathrin Light Chain
- 克隆位点
- SPM174
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CLTA/CLTB Primary Antibody
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anti-Clathrin, Light Chain A/B (CLTA/CLTB) (N-Term) antibody
CLTA/CLTB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CLC-1421 unconjugated
anti-Clathrin, Light Chain A/B (CLTA/CLTB) (C-Term) antibodyCLTA/CLTB 适用: 人, 小鼠, 大鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: HeLa cells. Placenta or Prostate Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))
- 别名
- CLTA and CLTB (CLTA/CLTB 产品)
- 别名
- lca antibody, zgc:73358 antibody, wu:fb61d08 antibody, wu:fj48d06 antibody, LCA antibody, clc antibody, DDBDRAFT_0169251 antibody, DDBDRAFT_0191305 antibody, DDB_0169251 antibody, DDB_0191305 antibody, clcb antibody, clathrin light chain A antibody, clathrin, light chain A antibody, Clathrin light chain antibody, clathrin light chain antibody, clathrin light chain B antibody, Clathrin light chain B antibody, CLTA antibody, clta antibody, clic-1 antibody, CLC1 antibody, clc antibody, CLTB antibody, clcb antibody, CNI00580 antibody, LOC5567495 antibody, CpipJ_CPIJ018056 antibody, PTRG_01988 antibody, PMAA_096950 antibody, SJAG_04517 antibody, TSTA_044110 antibody, PAS_chr1-4_0158 antibody
- 分子量
- 31-44kDa
- 基因ID
- 1211, 1212
- UniProt
- P09496, P09497
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