TOP1MT 抗体
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- 抗原 See all TOP1MT 抗体
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TOP1MT antibody is un-conjugated
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应用范围
- Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Staining Methods (StM)
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant full-length human TOP1MT protein
- 克隆位点
- TOP1MT-488
- 亚型
- IgG2b kappa
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anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibody
TOP1MT 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal TOP1MT-568 unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 470-550) antibodyTOP1MT 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 IHC, StM 宿主: 兔 Monoclonal TOP1MT-2883R unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 270-400) antibodyTOP1MT 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) antibodyTOP1MT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (Internal Region) antibodyTOP1MT 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 11-238) antibodyTOP1MT 适用: 小鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I, Mitochondrial (TOP1MT) (AA 1-238) antibodyTOP1MT 适用: 大鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Positive Control: MCF-7, A431 cells. Jurkat cell lysate. Heart, Skeletal Muscle, Brain or Fetal Liver tissue.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 浓度
- 200 μg/mL
- 缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- 有效期
- 24 months
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- 抗原
- TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
- 别名
- TOP1MT (TOP1MT 产品)
- 别名
- 2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
- 背景
- DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
- 分子量
- 70kDa
- 基因ID
- 116447
- UniProt
- Q969P6
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