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N-Cadherin 抗体 (Extracellular Domain)

Name: N-Cadherin 抗体 (Extracellular Domain)
Brand: antibodies-online
SKU: ABIN6939013
Availability: OutOfStock

CDH2 适用: 人 WB, IHC, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal 8C11 unconjugated

Western blot analysis of Heart tissue lysate using N-Cadherin Mouse Monoclonal Antibody (8C11). (N-Cadherin 抗体 (Extracellular Domain))

Formalin-fixed, paraffin-embedded Mouse Heart stained with N-Cadherin Mouse Monoclonal Antibody (8C11). (N-Cadherin 抗体 (Extracellular Domain))

IHC

SDS-PAGE Analysis Purified N-Cadherin Mouse Monoclonal Antibody (8C11). (N-Cadherin 抗体 (Extracellular Domain))

SDS

3 images

产品编号 ABIN6939013

发货至: 中国

Datasheet as PDF

抗原 See all N-Cadherin (CDH2) 抗体

N-Cadherin (CDH2) (Cadherin 2 (CDH2))
抗原表位
18

15

14

8

6

5

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Extracellular Domain
适用
138

92

67

21

16

14

8

4

3

3

3

3

2

2

2

2

2

1

1

1
人
宿主
92

53

4

4

2
小鼠
Compatible Secondaries
克隆类型
85

70
单克隆
标记
80

15

5

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This N-Cadherin antibody is un-conjugated
应用范围
110

52

38

38

25

22

17

14

13

13

11

8

4

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

特异性

Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

交叉反应（详细）

Human and Mouse. Others not known.

纯化方法

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

免疫原

Recombinant human N-cadherin extracellular domain

克隆位点

8C11

亚型

IgG1

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应用备注

Positive Control: HeLa or HUVEC cells. Human heart, pancreas or cerebral cortex (IHC).
Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blotting (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tis with1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

限制

仅限研究用
浓度

200 μg/mL

缓冲液

Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

有效期

24 months
抗原

N-Cadherin (CDH2) (Cadherin 2 (CDH2))

别名

CDH2 (CDH2 产品)

别名

CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody

背景

Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD,N-Cadherin / Cadherin-2 / CD325 (NCAD)
Cellular localisation: Cell surface

分子量

130-140kDa

基因ID

1000, 464829

UniProt

P19022

途径

Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane