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TOP1MT 抗体

TOP1MT 适用: 人 IF, WB, FACS, IHC (f) 宿主: 小鼠 Monoclonal TOP1MT-488 unconjugated
产品编号 ABIN6938633
发货至: 中国
  • 抗原 See all TOP1MT 抗体
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This TOP1MT antibody is un-conjugated
    应用范围
    • 10
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    Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    特异性
    DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
    交叉反应 (详细)
    Human.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Recombinant full-length human TOP1MT protein
    克隆位点
    TOP1MT-488
    亚型
    IgG2b kappa
    Top Product
    Discover our top product TOP1MT Primary Antibody
  • 应用备注

    Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7 or A431 cells. Jurkat cell lysate. Human heart, skeletal muscle, brain or fetal liver tissue.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))
    别名
    TOP1MT (TOP1MT 产品)
    别名
    2900052H09Rik antibody, TOP1 antibody, topoisomerase (DNA) I, mitochondrial antibody, DNA topoisomerase 1, mitochondrial antibody, DNA topoisomerase I mitochondrial antibody, TOP1MT antibody, Top1mt antibody
    背景
    DNA topoisomerase I, TOP1MT, Topoisomerase I mitochondrial, Type IB Topoisomerase,Topoisomerase I, Mitochondrial (TOP1MT)
    Cellular localisation: Cytoplasm (Mitochondria)
    分子量
    70kDa
    基因ID
    116447, 528574
    UniProt
    Q969P6
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