SPTA1 抗体 (AA 356-475)
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- 抗原 See all SPTA1 抗体
- SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))
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抗原表位
- AA 356-475
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SPTA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 特异性
- Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant human SPTA fragment (around aa356-475) (exact sequence is proprietary)
- 克隆位点
- SPTA1-1832
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product SPTA1 Primary Antibody
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- 应用备注
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL). Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K562, HeLa, Jurkat cells. Placenta or Tonsil.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))
- 别名
- SPTA1 (SPTA1 产品)
- 别名
- Spna1 antibody, AF093576 antibody, AI451697 antibody, Spna-1 antibody, ha antibody, ihj antibody, nmf4 antibody, sph antibody, EL2 antibody, HPP antibody, HS3 antibody, SPH3 antibody, SPTA antibody, spectrin, alpha, erythrocytic 1 antibody, spectrin alpha, erythrocytic 1 antibody, Spta1 antibody, SPTA1 antibody
- 背景
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Alpha I spectrin, Elliptocytosis 2 (EL2), Erythrocyte alpha spectrin, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha erythrocytic 1, SPH3, SPTA1,Spectrin Alpha 1 (Erythrocyte Marker)
Cellular localisation: Cytoplasmic and Cell Surface - 分子量
- 240kDa
- 基因ID
- 6708, 119825
- UniProt
- P02549
- 途径
- Regulation of Actin Filament Polymerization
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