Melanoma Marker (MART-1 + Tyrosinase + Gp100) 抗体
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- 抗原
- Melanoma Marker (MART-1 + Tyrosinase + Gp100)
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
- 特异性
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
- 纯化方法
- Purified by Protein A/G
- 免疫原
- Recombinant hMART-1 protein (DT101 & BC199), Extract of pigmented melanoma metastases from lymph nodes (HMB45), Recombinant tyrosinase protein (T311)
- 克隆位点
- DT101-BC199-T311-HMB45
- 亚型
- IgG2a kappa
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anti-Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody
适用: 人 IHC, ISt, StM 宿主: 小鼠 Monoclonal M2-7C10-M2-9E3-T311-HMB45 unconjugated
anti-Melanoma Marker (MART-1 + Tyrosinase + Gp100) antibody适用: 人 IHC, ISt, StM 宿主: 小鼠 Monoclonal DT101-BC199-T311-HMB45 unconjugated
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- 应用备注
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Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- 限制
- 仅限研究用
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- 缓冲液
- 10 mM PBS without BSA and without Azide.
- 储存液
- Azide free
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.
- 有效期
- 24 months
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- 抗原
- Melanoma Marker (MART-1 + Tyrosinase + Gp100)
- 分子量
- 20-22kDa (doublet) (MART-1): 90-100kDa (gp100), 70-80kDa (Tyrosinase)
- 基因ID
- 2315, 6490
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